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    Besides her own DNA, mother''s blood also contain small amount of fetal DNA. Therefore, it allows accurate screening for fetal Down syndrome using mother''s blood. If the baby has Down syndrome, the test will show increased amount of DNA from chromosome 21. 23DNA …

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    Digestion of Methylated DNA. DNA methylation is the process of transferring a methyl group from a donor molecule to either a cytosine or an adenine by DNA methyltransferases. Such methylation is the most common and abundant DNA modification process in living organisms. Three types of methylated bases are predominantly found in DNA: Other ...

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    Isotope effect may cause partial chromatographic separation of labeled (heavy) and unlabeled (light) isotopologue pairs. Together with a simultaneous matrix effect, this could lead to unacceptable accuracy in quantitative liquid chromatography-mass spectrometry

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     · Approximately 2 μg genomic DNA from A. rusticana were digested over night with Bsp143I, BsaWI, PsuI, XhoII, HindIII with the corresponding buffer in order to get fragments of 1-5 kb size. The digestion was stopped by heat inactivation of the enzymes, the fragmented DNA was precipitated with EtOH and the pellet was dissolved in 30 μL dH 2 O.

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    ObjectivesTo develop a novel screening method for DNAzymes targeting the LMP1 carboxy region.Study DesignTo design a method to screen special DNAzymes toward the Epstein-Barr virus (EBV)–associated... Skip to main content

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  • Technical Notes and Applications for Laboratory Work

    the meniscus of the screening solution in the reservoir. This avoids contamination via ''creeping'' of the screening solution by capillary action from the reservoir into the sample well (Fig. 10). For an overview of our hydrophobic plates see Appendix. forum No. 7 5 I 6

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    Infection Screening Ward Infectious Disease Beds (located in A4 & B4 wards) (A4 B4 )

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  • hisT is part of a multigene operon in Escherichia coli K-12. …

    The presence of the hisT gene on this plasmid was suggested by its ability to restore both production of PSUI enzymatic activity and suppression of amber mutations in a hisT mutant strain. A 2.3-kilobase HindIII-ClaI restriction fragment containing the hisT gene was subcloned into plasmid pBR322, and the resulting plasmid (designated psi 300) was mapped with restriction enzymes.

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  • Pregnancy-specific urinary incontinence in women with …

     · Results The overall prevalence rate of PSUI was 54.1 %, with prevalence rates of 43.3 % and 56.7 % in normoglycaemic and hyperglycaemic Brazilian pregnant women, respectively. Women with gestational hyperglycaemia had a higher amount of urine loss (p < 0.0027), frequency of UI (p < 0.0014), impact of UI on QoL (p < 0.0001), severity of UI (p = 0.0003) and total scores on the International ...

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    1.. (1)/: Serum/02- (Serum seperator tube Ⅱ) (2): 3 mL. (3) (): 1.HLA typing 2.HLA (),14。. 3.。. 2..

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